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KMID : 0350519930460020679
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Journal of Catholic Medical College
1993 Volume.46 No. 2 p.679 ~ p.687
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Effects of Thymopentin on Herpes Simplex Virus Infection in Human Fibroblast Monolayers
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Abstract
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Thymopentin is a suggestive treatment agent for herpes simplex virus infection. In previous studies thymopentin has been shown to induce changes in cell-mediated immunity. However, the effects of thymopentin on herpes simplex virus infection in
vitro
have not been investigated.
We studied the effect of thymopentin on herpes simplex virus infection in hyman fibroblast monolayers. Mononuclear cells were obtained form herpes simplex patients, and healthy adults. The cells were cultured in the presence of different
thymopentin
concentrations(10E5, 10E4, 10E3, 10E2, 10E1, 10E1, 10E-1, and 0 ng/ml). After the supernatant was harvested the fibroblast monolayers were incubated in the presence of different thymopentin concentrations(10E5-10E-1 and 0 ng/ml) or supernatant
for
30
minutes. After incubation the fibroblast monolayers were inoculated with herpes simplex virus. The number of herpes simplex virus infected cells was determined by plaque assay and immunofluorescence.
@ES The results were as follows :
@EN 1. Thymopentin increased the number of plaques 43.4% at 10E ng/ml and 33.0% at 10E1 ng/ml, and immunofluorescent staining cells 2.93% and 2.67% respectively.
2. Mononuclear cell supernatant from herpes simplex patients decreased the number of plaques 12.3%, and immunofluorescent staining cells 3.63% at 10E3 ng/ml thymopentin concentration.
3. Mononuclear cell supernatant from healthy adults decreased the number of plaques 5.7% at 10E5, 10E3, and 10E2 ng/ml thymopentin concentrations.
In conclusion, thymopentin increased herpes simplex virus infection in human fibroblast monolayers cultured at 10E3 ng/ml(P<0.05). Mononuclear cell supernatant from herpes simplesx patients cultured at 10E3 ng/ml thymopentin decreased herpes
simplex
virus infection in hyman fibroblast monolayers.
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KEYWORD
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